Mapping myosin light chains by immunoelectron microscopy. Use of anti-fluorescyl antibodies as structural probes.
نویسندگان
چکیده
منابع مشابه
Mapping myosin light chains by immunoelectron microscopy. Use of anti- fluorescyl antibodies as structural probes
The two classes of light chains in vertebrate fast muscle myosin have been selectively labeled with the thiol specific reagent 5-(iodoacetamido) fluorescein to determine their location in the myosin head. The alkali light chains (A1 and A2) were labeled at a single cysteine residue near the COOH terminus, whereas the regulatory light chain (LC2) was reacted at either cysteine 125 or 154. The tw...
متن کاملUse of anti-idiotypic antibodies as cell-surface receptor probes.
Anti-idiotypic antibodies have been raised against antibodies to retinol-binding protein (RBP) and to insulin. After absorption the anti-idiotypic antibodies recognized the antigen-combining sites of the antibodies used as the immunogen but of no other antibodies. Some of the anti-idiotypic antibodies raised against antibodies to RBP bound specifically to rat intestine epithelial cells, which h...
متن کاملSizing of protein A-colloidal gold probes for immunoelectron microscopy
Gold particles in colloidal solutions often vary considerably in size. The finest sols (diameter less than 15 nm), especially, are very heterogeneous, as is indicated by coefficients of variance (CV) of 25-35%. We have complexed staphylococcal protein A with gold particles (PA/Au) and then fractionated the preparations by glycerol or sucrose gradient centrifugation into very homogeneous subfrac...
متن کاملGap junction communication channel: peptides and anti-peptide antibodies as structural probes.
856 benzodiazepine photoaffinity labelling site on the GABA, receptor a 1 subunit [ 121. Further, it should be pointed out that an alternative approach to synthetic peptides as antigens for directed antibody production is the use of bacterially produced subunit fragments, and indeed this strategy has been adopted for the GAHA, receptor [13]. In general these antigens, although probably less exp...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1989
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.109.4.1549